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Determination of Kresoxim-Methyl in Water and in Grapes by High-Performance Liquid Chromatography (HPLC) Using Photochemical-Induced Fluorescence and Dispersive Liquid-Liquid Microextraction (DLLME).
- Source :
-
Analytical Letters . 2020, Vol. 53 Issue 14, p2202-2221. 20p. 1 Diagram, 3 Charts, 8 Graphs. - Publication Year :
- 2020
-
Abstract
- A high-performance chromatographic method was developed to determine the fungicide kresoxim-methyl. Off-line photochemical derivatization was used to induce the formation of a stable and fluorescent product since the fungicide does not present natural fluorescence. Intense fluorescence at 370/430 nm was achieved by treating the analyte in solution at pH 6 to ultraviolet light for 45 s. The chromatographic conditions included isocratic elution with 50/50% (v/v) acetonitrile/water and the photochemical product appeared at a retention time of 7.2 min. The short and long term stabilities of the photoproduct were evaluated and variation of less than 5% was achieved. The limits of detection in water samples and in grapes samples were 0.019 mg kg−1 and 0.065 mg kg−1 of kresoxim- methyl residue, respectively. The linear response covered three orders of magnitude up to 10.6 mg kg−1 of kresoxim-methyl. The robustness was evaluated through a Box–Behnken experimental design showing the insignificance of all factors and their interactions. The potential interference of tebuconazole for the determination of kresoxim-methyl was studied. The use of the dispersive liquid-liquid microextraction (DLLME) allowed recoveries between 80% and 101% depending on concentration with the minimum generation of waste products. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032719
- Volume :
- 53
- Issue :
- 14
- Database :
- Academic Search Index
- Journal :
- Analytical Letters
- Publication Type :
- Academic Journal
- Accession number :
- 144474860
- Full Text :
- https://doi.org/10.1080/00032719.2020.1733589