二十味沉香丸对卒中后 PTSD 模型大鼠行为学及 5-HT1AR 的 调节作用探析.

Objectives: To explore the effect of the Tibetan medicine ErShiwei Chenxiang Pill on the regulation of rat behavior and 5-HT1AR in PTSD model after stroke. Methods: 48 rats were randomly divided into sham operation group, model group, Ershiwei Chenxiang pill group and paroxetine group. There are 12 rats in each group. The rats that did not die at the observation time after modeling were excluded from this study, and the qualified rats were screened out and the animals were supplemented by the principle of random sampling. After the model was established, the corresponding drugs or saline were given intragastrically every day. 2 weeks later, the behavioral changes of the remaining rats in each group were detected by open field test. Each group has its own The changes of 5-HT1AR gene and protein in hippocampus were detected by real-time fluorescence quantitative PCR and Western blot, respectively. Results: Compared with the sham group, data of the model group, the total distance (P<0.01), the single maximum movement distance (P<0.05), times of spanning lattice (P <0.01), vertical activity (P <0.01), the vertical time (P <0.01), the time of modification (<0.01) and the number of modification (P <0.05) were of statistical significance. The expression level of 5-HT1AR gene was of statistical significance (<0.05), and the level of protein expression was decreased. Compared with the model group, the data of the ESWCX, the total distance (P<0.05), times of spanning lattice (P<0.05) the vertical time (P <0.05) and vertical activity (P<0.01), and the expression of 5-HT1AR gene (P<0.05) and protein level were increased. Compared with the model group, paroxetine group had a statistically significant difference in the number of times of penetration (P <0.05), the total distance (P<0.05), and the vertical time (P<0.05). The gene of 5-HT1AR (P <0.01) and the level of protein expression were increased. Conclusion: This experiment has successfully copied the PTSD model after stroke, and the selected Chinese medicine Ershiwei Chenxiang pill can be used to improve the anxiety-like behavior of the PTSD model in the post-stroke PTSD model, the utility is similar to that of the western medicine paroxetine, and the mechanism of action may be related to the regulation of 5-HT1AR. [ABSTRACT FROM AUTHOR]