落叶松针叶化学成分研究.

To study on chemical constituents and bioactivity of Larix gmelinii needles, separation and purification of ethyl acetate extract from Larix gmelinii needles were carried out by silica gel column chromatography，semi-preparative high performance liquid chromatography，and re-crystallization. The structures of isolated compounds wrere identified by analysis of spectra of MS，NMR，and compared wdth the reported conferences. The antibacterial activity of the extract ■was also tested. The results 'were as follo'ws: Compounds 1-15 wrere isolated from the ethyl acetate extract and identified as Larixol ( 1)，( 2R)-5, 4,-dihydroxy-6-methyl-7-methoxy flavone ( 2 ), 2', 4,-dihydroxy-4, 6,-dimethoxydihydro- chalcone (3) , 2,,4-dihydroxy-4,,6,-dimethoxy-chalcone (4) , 2,,4,-dihydroxy-4,6,-dimethoxy-chalcone (5) , isorham- netin (6)，4'，5-dihydroxy-7-methoxy-8-methyl flavone (7)， kaempferol (8)， p-sitosterol (9)， stigmasterol (10)， daucosterol (11)，vanillic acid (12)，hydroxyl-benzoic acid (13)， dimethyl - matairesinol (14)，and 15-nonacosanol (15). Compounds 2，4，5，and 7 were isolated from the genus Pinus for the first time. The result of antibacterial activity indicated that the inhibition rates of ethyl acetate extract to Escherichia coli，Bacillus subtilis，B. cereus，and Staphylococcus aureus were 55%-70%，53%-72%，61%-71%，33%-65%，respectively，at the concentration range of 5-100 mg • mL 1. The results of this study wall provide a theoretical basis for further study on the chemical composition and pharmacological activity of Larix gmelinii. [ABSTRACT FROM AUTHOR]