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Polyethylene glycol molecular crowders enhance the catalytic ability of bimolecular bacterial RNase P ribozymes.
- Source :
-
Nucleosides, Nucleotides & Nucleic Acids . 2020, Vol. 39 Issue 5, p715-729. 15p. - Publication Year :
- 2020
-
Abstract
- The modular structure of bacterial ribonuclease P (RNase P) ribozymes, which recognize tertiary structures of precursor tRNAs (pre-tRNAs) to cleave their 5′ leader sequence, can be dissected physically into the two structured domain RNAs (S-domain and C-domain). Separately prepared S-domain RNA and C-domain RNA assemble to form bimolecular forms of RNase P ribozymes. We analyzed the effects of polyethylene glycols (PEGs) on pre-tRNA cleavage catalyzed by bimolecular RNase P ribozymes to examine the effects of molecular crowding on the reaction. PEG molecular crowders significantly enhanced the activities of bimolecular RNase P ribozymes, some of which were hardly active without PEGs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15257770
- Volume :
- 39
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Nucleosides, Nucleotides & Nucleic Acids
- Publication Type :
- Academic Journal
- Accession number :
- 143848329
- Full Text :
- https://doi.org/10.1080/15257770.2019.1687909