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A circRNA derived from linear HIPK3 relieves the neuronal cell apoptosis in spinal cord injury via ceRNA pattern.
- Source :
-
Biochemical & Biophysical Research Communications . Jul2020, Vol. 528 Issue 2, p359-367. 9p. - Publication Year :
- 2020
-
Abstract
- Spinal cord injury (SCI) is a severe disable symptom and has posed a great health threat to many people. Circ-HIPK3 has been reported to modulate the biological behavior of neuronal cells. Thence, in this study, we explored the mechanism of circ-HIPK3 in affecting functions of neuronal cell in SCI. SCI rat model was constructed to evaluate the apoptosis condition of spinal cord tissue. Meanwhile, 100 μM of CoCl 2 was used to treat AGE1.HN and PC12 cells to induce in vitro SCI model. Functional assays were implemented to investigate the apoptosis of AGE1.HN and PC12 cells. RNase R and Act D treatment were both conducted to verify the circular character of circ-HIPK3. In this study, circ-HIPK3 was found lowly expressed in SCI rat models and AGE1.HN and PC12 cells induced by 100uM of CoCl 2. Meanwhile, inhibited circ-HIPK3 or overexpressed circ-HIPK3 could separately elevate or reduce the apoptosis of AGE1.HN and PC12 cells. Moreover, circ-HIPK3 was identified as the ceRNA against miR-558 to up-regulate DPYSL5. Circ-HIPK3/miR-558/DPYSL5 axis modulated the apoptosis of AGE1.HN and PC12 cells in SCI. In conclusion, circ-HIPK3 relieves the neuronal cell apoptosis through regulating miR-588/DPYSL5 axis in SCI. • Circ-HIPK3 was downregulated in SCI rat model and CoCl 2 induced neuronal cells. • Circ-HIPK3 could suppress the apoptosis of neuronal cells. • Circ-HIPK3 regulates DPYSL5 by acting as a ceRNA. • Circ-HIPK3 regulates neuronal cell apoptosis in SCI via axon guidance pathway. [ABSTRACT FROM AUTHOR]
- Subjects :
- *APOPTOSIS
*SPINAL cord injuries
*CIRCULAR RNA
*CELL physiology
*SPINAL cord
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 528
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 143700607
- Full Text :
- https://doi.org/10.1016/j.bbrc.2020.02.108