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Rapid Analysis of Four Amphetamines in Urine by Self-Made Pipette-Tip Solid-Phase Extraction Followed by GC-MS/MS.
- Source :
-
Journal of Chromatographic Science . Jul2020, Vol. 58 Issue 6, p569-575. 7p. - Publication Year :
- 2020
-
Abstract
- A simple and rapid pipette-tip solid-phase extraction (PT-SPE) procedure with derivatization prior to gas chromatography triple quadrupole mass spectrometry analysis is developed for the simultaneous determination of amphetamine (AMP), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA) in urine samples. The PT-SPE procedure using self-made sorbent could extract drugs within 6 min from 100-μL urine samples, requiring low solvent-consumption (<2.0 mL). Besides, the self-made pipette tip could be reused at least five times. Under optimized conditions, the recoveries of four amphetamines at spiked levels (low, medium and high) ranged from 87.7 to 110.4%, with relative standard deviations < 9.5%. The limit of detections and limit of quantifications for AMP, MA, MDA and MDMA were in the range of 2.52–8.25 ng⋅mL−1 and 8.4–27.5 ng⋅mL−1, respectively. Validation results show that the proposed method is suitable for the quantitation of amphetamines and has been successfully applied in the urine samples of suspected drug abusers. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00219665
- Volume :
- 58
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatographic Science
- Publication Type :
- Academic Journal
- Accession number :
- 143636359
- Full Text :
- https://doi.org/10.1093/chromsci/bmaa018