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Effects of infectious bursal disease virus infection on interferon and antiviral gene expression in layer chicken bursa.
- Source :
-
Microbial Pathogenesis . Jul2020, Vol. 144, pN.PAG-N.PAG. 1p. - Publication Year :
- 2020
-
Abstract
- Layer chickens were artificially challenged with infectious bursal disease virus (IBDV), and the kinetics of IFN-λ and antiviral genes in the bursa were explored using quantitative real-time PCR. Data showed that after the chickens were infected with IBDV, the virus load in the bursa of the Fabricius peaked at 96 h and gradually decreased. The relative mRNA expression levels of IFN-λ and antiviral genes (zinc-finger antiviral protein ZAP , interferon alpha-inducible protein 6 IFI6 , laboratory of genetics and physiology 2 LGP2 , virus inhibitory protein Viperin , and Mx) of the infected group dramatically increased at 24–168 h compared with those of the negative-infected group. Furthermore, the ZAP mRNA expression peaked at 24 h (3.97-fold). The Viperin mRNA transcript level was highest at 48 h (384.60-fold). The mRNA expression levels of IFI6 (96.31-fold), LGP2 (18.29-fold), and Mx (88.85-fold) peaked at 72 h, and that of IFN-λ was most remarkable at 96 h (2978.81-fold). Furthermore, the ZAP change rule was significantly positively correlated with the change rule of the IBDV load. The mRNA expression levels of IFN-λ and antiviral genes (ZAP , IFI6 , LGP2 , Viperin , and Mx) increased as the virus expression increased and then decreased. These results further corroborated that the IBDV infection seriously interfered with the chicken's innate immune response. • The expression levels of IFN-λ , ZAP , IFI6 , LGP2 , Viperin , and Mx were remarkably increased along with different IBDV loads. • Expression of IFN-λ , ZAP , IFI6 , LGP2 , Viperin , and Mx mRNA peaked at different time points, some early on, some later. • The kinetics of ZAP relative expression level had a significant positive correlation with the change in IBDV load. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08824010
- Volume :
- 144
- Database :
- Academic Search Index
- Journal :
- Microbial Pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 143558911
- Full Text :
- https://doi.org/10.1016/j.micpath.2020.104182