Improvement of adhesion properties of enzyme‐loaded coating on random packing in transesterification.

The stability of immobilized enzyme on packing is an important factor to limit the development of enzyme‐catalyzed reaction distillation. The modified sol loaded with Candida antarctica lipase B (CALB) was fixed on random packing (316 L stainless steel wire mesh) by spraying method to form an enzyme‐loaded coating, which was applied to catalyze the esterification of ethyl acetate and n‐butanol. The addition of 1‐[3‐(trimethoxysilyl)propyl]ureido (UPS) enhanced the stability of the CALB, and the mechanism of its combination was explored from a microscopic perspective. And then, it was confirmed by scratch resistance test that adhesion of enzyme‐loaded coating in packing was improved 19%. The esterification reaction showed that the addition of UPS had little effect on the conversion rate while improving the stability of the coating. These results suggest that the effect of UPS treatment on the enzyme‐loaded coating performance significantly enhanced the long‐term effect of enzyme‐loaded packing. [ABSTRACT FROM AUTHOR]