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Do fetal extravillous trophoblasts circulate in maternal blood postpartum?
- Source :
-
Acta Obstetricia et Gynecologica Scandinavica . Jun2020, Vol. 99 Issue 6, p751-756. 6p. - Publication Year :
- 2020
-
Abstract
- <bold>Introduction: </bold>Circulating fetal extravillous trophoblasts may offer a superior alternative to cell-free fetal DNA for noninvasive prenatal testing. Cells of fetal origin are a pure source of fetal genome; hence, unlike the cell-free noninvasive prenatal test, the fetal cell-based noninvasive prenatal test is not expected to be affected by maternal DNA. However, circulating fetal cells from previous pregnancies may lead to confounding results.<bold>Material and Methods: </bold>To study whether fetal trophoblast cells persist in maternal circulation postpartum, blood samples were collected from 11 women who had given birth to a boy, with blood sampling at 1-3 days (W0), 4-5 weeks (W4-5), around 8 weeks (W8) and around 12 weeks (W12) postpartum. The existence of fetal extravillous trophoblasts was verified either by X and Y chromosome fluorescence in situ hybridization analysis or by short tandem repeat analysis. To exclude technological bias in isolating fetal cells, blood samples were also collected from 10 pregnant women between a gestational age of 10 and 14 weeks, the optimal time frame for cell-based noninvasive prenatal test sampling. All the samples were processed according to protocols established by ARCEDI Biotech for fetal extravillous trophoblast enrichment and isolation.<bold>Results: </bold>Fetal extravillous trophoblasts were found in all the 10 samples from pregnant women between a gestational age of 10 and 14 weeks. However, only 4 of 11 blood samples taken from women at 1-3 days postpartum rendered fetal extravillous trophoblasts, and only 2 of 11 samples rendered fetal extravillous trophoblasts at 4 weeks postpartum.<bold>Conclusions: </bold>In this preliminary dataset on few pregnancies, none of the samples rendered any fetal cells at or after 8 weeks postpartum, showing that cell-based noninvasive prenatal testing based on fetal extravillous trophoblasts is unlikely to be influenced by circulating cells from previous pregnancies. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SHORT tandem repeat analysis
*FLUORESCENCE in situ hybridization
*CHORIONIC villus sampling
*Y chromosome
*PRENATAL diagnosis
*RESEARCH
*TROPHOBLAST
*DNA
*CYTOMETRY
*RESEARCH methodology
*EVALUATION research
*COMPARATIVE studies
*PUERPERIUM
*QUESTIONNAIRES
*MENTAL health surveys
*RESEARCH funding
*POLYMERASE chain reaction
Subjects
Details
- Language :
- English
- ISSN :
- 00016349
- Volume :
- 99
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Acta Obstetricia et Gynecologica Scandinavica
- Publication Type :
- Academic Journal
- Accession number :
- 143431270
- Full Text :
- https://doi.org/10.1111/aogs.13880