施今墨对药配方对2型糖尿病大鼠肝脏胰岛素抵抗及 PI3K/AKT/GSK-3β 信号通路的影响.

Objective To observe the effects of Shijinmo herbal-pair formula on the liver glycogen content, and PI3K/AKT/GSK-3β signaling pathway in type 2 diabetic rats, and to explore its action mechanism in reducing the insulin resistance. Methods Six rats were randomly selected from 40 rats and given common diet, and the rest were given highfat/sugar diet combined with intraperitoneal injection of low-dose streptozotocin to establish type 2 diabetic models. Twentyfour diabetic rats were randomly divided into the model control group, and low-dose, medium-dose and high-dose Shijinmo herbal-pair formula groups, with 6 rats in each group. The rats in the normal control group and the model control group were given pure water ( 1. 5 mL/100 g), and the rats in the low-dose, medium-dose and high-dose Shijinmo herbal-pair formula groups were given the corresponding drugs (equals to the original herbs of 5. 8, 11. 6 and 23. 2 glkg) for 8 weeks. Blood glucose was measured with glucose oxidase method. The level of serum insulin was measured by ELISA. The HomaIR formula was used to calculate IR index. The hepatic glycogen content was measured by the glycogen assay kit; the protein expression levels of PI3K, AKT and GSK-3β, and their phosphorylation levels were analyzed by Western blotting. Results Compared with the normal control group, the blood glucose level increased, IRI increased, liver glycogen content decreased, the protein express levels of PI3 K and AKT, and their phosphorylation levels decreased, GSK-3β protein expression increased, but its phosphorylation level decreased in the model control group (all P < 0. 05) . Compared with the model control group, the blood glucose level and IRI value were significantly reduced, the glycogen content in the liver increased in type 2 diabetic rats after the treatment of low-dose Shijinmo herbal-pair formula group. The low-dose Shijinmo herbal-pair formula increased the protein expression levels of PI3K and AKT and their phosphorylation levels, and decreased the protein level of GSK-3β but enhanced its phosphorylation level (all P <0. 05). The above indicators were not significantly improved in the medium-dose and high-dose Shijinmo herbal-pair formula groups. Conclusion Shijinmo herbal-pair formula has hypoglycemic effect through enhancing the insulin signal transduction pathway of PBK-AKT-GSK3β, thereby increasing the glycogen content in hepatocytes and reducing the degree of IR. [ABSTRACT FROM AUTHOR]