Impacts of nitrogen loads on the water and biota in a karst river (Loue River, France).

We measured nitrate in water (n = 4762) and nitrogen stable isotopes (δ15N) (n = 353) of macroalgae and macroinvertebrates at different sites in a French karst river (Loue River) which has moderate nitrogen loading (~ 23 kg nitrogen ha−1 year−1). The main objective was to estimate whether nitrate in water and nitrogen stable isotopes of the biota could allow identification of the spatial and temporal variations in agriculture-related nitrogen losses to the river. Highest nitrate concentrations (> 90% quantile) increased significantly over the last three decades but mean nitrate concentrations did not change significantly. Nitrate and biota δ15N values tended to increase from upstream to downstream, although the δ15N values decreased in the most downstream sites. Generalized additive mixed models allowed the identification of clear matching annual patterns of nitrate and biota δ15N values characterized by recurrent autumn to winter maxima, supporting that agricultural nitrogen export and its assimilation within the river biota would mostly occur during the autumn and winter seasons. Overall, our results highlight the high vulnerability of karst river to nitrogen-related eutrophication. [ABSTRACT FROM AUTHOR]