菊芋果聚糖合成酶基因1-SST启动子克隆与活性研究.

【Objective】This paper aimed to isolate and conduct bioinformatics analysis of the sucrose: sucrose-1-fructosyltransferase(1-SST) gene from Jerusalem artichoke. 【Method】 The Jerusalem artichoke 1-SST by Tail-PCR was isolated. A sequence of 1816 bp upstream of the gene start codon(SSTP). Bioinformatics analysis of SSTP sequences was performed by PLANTCARE. 【Result】 A total of 140 cis-acting elements were detected in the SSTP sequence. In addition to the 44 TATA-box and 31 CAAT-box elements of the promoter, there were 65 cis-independent expressions of 1-SST gene expression. Acting element of the 65 cis-acting elements, 23 cis-acting elements were capable of predicting their function. There were 13 light-regulating, two components of abscisic acid, anaerobic process and low temperature stress, and one component of salicylic acid, stress resistance, meristem expression and endosperm expression. The identification of these cis-acting elements provided a theoretical basis for 1-FFT participation in the corresponding biological processes. The CaMV 35 S promoter of pCAMBIA1301 vector was replaced with the sequence of SSTP, 1/2 SSTP and 1/4 SSTP, respectively, and transiently expressed in tobacco leaves. After GUS staining, SSTP, 1/2 SSTP and 1/4 SSTP were all driven to drive GUS. Promoter activity of gene expression, and activity is comparable to CaMV35 S. 【Conclusion】 It is preliminarily estimated that the promoter core region of 1-SST gene is within 400 bp upstream of the start codon. [ABSTRACT FROM AUTHOR]