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Kinesin KIF18A is a novel PUM-regulated target promoting mitotic progression and survival of a human male germ cell line.

Authors :
Smialek, Maciej Jerzy
Kuczynska, Bogna
Ilaslan, Erkut
Janecki, Damian Mikolaj
Sajek, Marcin Piotr
Kusz-Zamelczyk, Kamila
Jaruzelska, Jadwiga
Source :
Journal of Cell Science. Apr2020, Vol. 133 Issue 7, preceding p1-8. 9p.
Publication Year :
2020

Abstract

Regulation of proliferation, apoptosis and cell cycle is crucial for the physiology of germ cells. Their malfunction contributes to infertility and germ cell tumours. The kinesin KIF18A is an important regulator of those processes in animal germ cells. Post-transcriptional regulation of KIF18A has not been extensivelyexplored.Owing to the presence of PUM-binding elements (PBEs), KIF18A mRNA is a potential target of PUM proteins, where PUM refers to Pumilio proteins, RNAbinding proteins that act in post-transcriptional gene regulation. We conducted RNA co-immunoprecipitation combined with RT-qPCR, as well as luciferase reporter assays, by applying an appropriate luciferase construct encoding wild-type KIF18A 3'-UTR, upon PUM overexpression or knockdown in TCam-2 cells, representing human male germ cells. We found that KIF18A is repressed by PUM1 and PUM2. To study how this regulation influences KIF18A function, an MTS proliferation assay, and apoptosis and cell cycle analysis using flow cytometry, was performed upon KIF18A mRNA siRNA knockdown. KIF18A significantly influences proliferation, apoptosis and the cell cycle, with its effects being opposite to PUM effects. Repression by PUM proteins might represent one of mechanisms influencing KIF18A level in controlling proliferation, cell cycle and apoptosis in TCam-2 cells. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00219533
Volume :
133
Issue :
7
Database :
Academic Search Index
Journal :
Journal of Cell Science
Publication Type :
Academic Journal
Accession number :
142872931
Full Text :
https://doi.org/10.1242/jcs.240986