虎杖提取物对人胰腺癌细胞系 Panc-1 增殖与凋亡的影响.

Objective: The effect of Polygonum cuspidatum extract on Pancreatic cancer cell line Panc-1 was investigated by extracting Polygonum cuspidatum extracts into human pancreatic cancer cell proliferation and apoptosis phenotype. Methods: Different concentrations(0, 10, 50, 100, 150, 200 μg/m L) of Polygonum cuspidatum extract were prepared, and each concentration of Polygonum cuspidatum extract was added to the human pancreatic cancer Panc-1 cell line to be treated for 24 h. The proliferation activity of the cell line Panc-1 was detected by CCK-8(cell counting kit-8) method; the human pancreatic cancer cell line Panc-1 was treated with 100 μg/m L Polygonum cuspidatum extract for 24 h, and then flow cytometry was used( FCM) was used to detect the cell cycle and apoptosis distribution; 100 μg/m L Polygonum cuspidatum extract was used to treat human pancreatic cancer cell line Panc-1 for 24 h, and the total RNA and total protein were extracted. Subsequent real-time PCR and Western blot were used to detect human pancreatic cancer.Transcription and translation levels of the cell line Panc-1 proliferation marker genes PCNA, CDK2 and apoptosis marker genes BAD,BAX. Results: The results of CCK-8 showed that the inhibition rate of Polygonum cuspidatum extract on the proliferation of human pancreatic cancer cell line Panc-1 increased with concentration. The results of flow cytometry showed that Polygonum cuspidatum extract inhibited the proliferation of human pancreatic cancer cells and promoted its apoptosis. The results of real-time PCR and Western blot showed The extract of Polygonum cuspidatum can decrease the expression of PCNA and CDK2 in human pancreatic cancer cell proliferation, and the expression of apoptosis marker gene BAD and BAX increased. Conclusion: Polygonum cuspidatum extract can inhibit the proliferation and promote apoptosis of human pancreatic cancer cell line Panc-1 [ABSTRACT FROM AUTHOR]