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Copper promotes sheep pancreatic duct organoid growth by activation of an antioxidant protein 1-dependent MEK-ERK pathway.
- Source :
-
American Journal of Physiology: Cell Physiology . Apr2020, Vol. 318 Issue 4, pC806-C816. 11p. - Publication Year :
- 2020
-
Abstract
- Proper amounts of copper supplemented in livestock feed improve the physical growth and traits of farm animals. The pancreas is an important organ with both exocrine and endocrine portions. To investigate the role and mechanism of copper in the sheep pancreas, we first established sheep pancreatic duct organoids (sPDOs). We found that an appropriate amount of copper benefited the formation and growth of sPDOs, whereas excess or deficient copper damaged sPDOs. We found that the proliferation-stimulating effect of copper was related to the copper chaperone antioxidant protein 1 (ATOX1)-dependent activation of MEK-ERK1/2 signaling. Atox1 knockdown suppressed the cell proliferation of sPDOs, even in the presence of the MEK activator. These results indicate that moderate concentrations of copper promote sPDO growth through ATOX1-regulated cell proliferation by activation of MEK-ERK. Moreover, our study indicates that organoids may be a useful model to study organ growth mechanisms in livestock. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PANCREATIC duct
*COPPER
*MOLECULAR chaperones
*SHEEP
Subjects
Details
- Language :
- English
- ISSN :
- 03636143
- Volume :
- 318
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- American Journal of Physiology: Cell Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 142568855
- Full Text :
- https://doi.org/10.1152/ajpcell.00509.2019