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Rapid and Simple Species Identification of Cicada Exuviae Using COI-Based SCAR Assay.

Rapid and Simple Species Identification of Cicada Exuviae Using COI-Based SCAR Assay.

Authors :
Noh, Pureum
Kim, Wook Jin
Song, Jun-Ho
Park, Inkyu
Choi, Goya
Moon, Byeong Cheol
Source :
Insects (2075-4450). Mar2020, Vol. 11 Issue 3, p168. 1p.
Publication Year :
2020

Abstract

Cicadidae periostracum (CP), the medicinal name of cicada exuviae, is well-known insect-derived traditional medicine with various pharmacological effects, e.g., anticonvulsive, anti-inflammatory, antitussive, and anticancer effects; it is also beneficial for the treatment of Parkinson's disease. For appropriate CP application, accurate species identification is essential. The Korean pharmacopoeia and the pharmacopoeia of the People's Republic of China define Cryptotympana atrata as the only authentic source of CP. Species identification of commercially distributed CP based on morphological features, however, is difficult because of the combined packaging of many cicada exuviae in markets, damage during distribution, and processing into powder form. DNA-based molecular markers are an excellent alternative to morphological detection. In this study, the mitochondrial cytochrome c oxidase subunit I sequences of C. atrata, Meimuna opalifera, Platypleura kaempferi, and Hyalessa maculaticollis were analyzed. On the basis of sequence alignments, we developed sequence-characterized amplified-region (SCAR) markers for efficient species identification. These markers successfully discriminated C. atrata from the three other cicada species, and detected the adulteration of market CP samples. This SCAR assay is a rapid, simple, cheap, reliable, and reproducible method for species identification, regardless of sample form and status, and contributes to CP quality control. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20754450
Volume :
11
Issue :
3
Database :
Academic Search Index
Journal :
Insects (2075-4450)
Publication Type :
Academic Journal
Accession number :
142565631
Full Text :
https://doi.org/10.3390/insects11030168