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Cohesin Removal Reprograms Gene Expression upon Mitotic Entry.

Cohesin Removal Reprograms Gene Expression upon Mitotic Entry.

Authors :
Perea-Resa, Carlos
Bury, Leah
Cheeseman, Iain M.
Blower, Michael D.
Source :
Molecular Cell. Apr2020, Vol. 78 Issue 1, p127-127. 1p.
Publication Year :
2020

Abstract

As cells enter mitosis, the genome is restructured to facilitate chromosome segregation, accompanied by dramatic changes in gene expression. However, the mechanisms that underlie mitotic transcriptional regulation are unclear. In contrast to transcribed genes, centromere regions retain transcriptionally active RNA polymerase II (Pol II) in mitosis. Here, we demonstrate that chromatin-bound cohesin is necessary to retain elongating Pol II at centromeres. We find that WAPL-mediated removal of cohesin from chromosome arms during prophase is required for the dissociation of Pol II and nascent transcripts, and failure of this process dramatically alters mitotic gene expression. Removal of cohesin/Pol II from chromosome arms in prophase is important for accurate chromosome segregation and normal activation of gene expression in G1. We propose that prophase cohesin removal is a key step in reprogramming gene expression as cells transition from G2 through mitosis to G1. • Mitotic centromere transcription requires cohesin • Prophase cohesin removal releases elongating Pol II and nascent RNA from chromatin • Chromatin release of Pol II/nascent RNA facilitates accurate chromosome segregation • The prophase pathway ensures G2/G1 gene expression reprograming across mitosis During mitosis, chromosomes condense and transcription is silenced to facilitate chromosome segregation. However, centromeres are transcribed during mitosis. Perea-Resa et al. find that mitotic cohesin regulation is important for mitotic transcriptional silencing, mitotic centromere transcription, and transcriptional restart in G1. Coupled cohesin/transcription dynamics ensures chromosome segregation and gene expression reprograming across mitosis. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10972765
Volume :
78
Issue :
1
Database :
Academic Search Index
Journal :
Molecular Cell
Publication Type :
Academic Journal
Accession number :
142498822
Full Text :
https://doi.org/10.1016/j.molcel.2020.01.023