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The Effects of Yuan-Zhi Decoction and Its Active Ingredients in Both In Vivo and In Vitro Models of Chronic Cerebral Hypoperfusion by Regulating the Levels of Aβ and Autophagy.
- Source :
-
Evidence-based Complementary & Alternative Medicine (eCAM) . 2/24/2020, Vol. 2020, p1-16. 16p. 1 Chart, 11 Graphs. - Publication Year :
- 2020
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Abstract
- Chronic cerebral hypoperfusion (CCH) is closely related to the occurrence of Alzheimer's disease (AD) in the elderly. CCH can induce overactivation of autophagy, which increases the deposition of amyloid-β (Aβ) plaques in the brain, eventually impairing the cognitive function. Yuan-Zhi decoction (YZD) is a traditional Chinese medicine (TCM) formulation that is used to treat cognitive dysfunction in the elderly, but the specific mechanism is still unclear. In this study, we simulated CCH in a rat model through bilateral common carotid artery occlusion (BCCAO) and treated the animals with YZD. Standard neurological tests indicated that YZD significantly restored the impaired cognitive function after BCCAO in a dose-dependent manner. Furthermore, YZD also decreased the levels of Aβ aggregates and the autophagy-related proteins ATG5 and ATG12 in their hippocampus. An in vitro model of CCH was also established by exposing primary rat hippocampal neurons to hypoxia and hypoglycemia (H-H). YZD and its active ingredients increased the survival of these neurons and decreased the levels of Aβ1-40 and Aβ1-42, autophagy-related proteins Beclin-1 and LC3-II, and the APP secretases BACE1 and PS-1. Finally, both Aβ aggregates showed a positive statistical correlation with the expression levels of the above proteins. Taken together, YZD targets Aβ, autophagy, and APP-related secretases to protect the neurons from hypoxic-ischemic injury and restore cognitive function. [ABSTRACT FROM AUTHOR]
- Subjects :
- *AUTOPHAGY
*ANIMAL experimentation
*HYPOXEMIA
*ARTERIAL occlusions
*CAROTID artery diseases
*CEREBRAL circulation
*CEREBROVASCULAR disease
*COGNITION
*COGNITION disorders
*DOSE-effect relationship in pharmacology
*GENE expression
*HERBAL medicine
*HIPPOCAMPUS (Brain)
*HYPOGLYCEMIA
*NEUROPSYCHOLOGICAL tests
*CHINESE medicine
*MEMBRANE proteins
*MOLECULAR structure
*NERVE tissue proteins
*NEURONS
*PROTEINS
*PROTEOLYTIC enzymes
*RATS
*CELL survival
*IN vitro studies
*IN vivo studies
*DISEASE complications
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Volume :
- 2020
- Database :
- Academic Search Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 142473002
- Full Text :
- https://doi.org/10.1155/2020/6807879