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A complete route for biodegradation of potentially carcinogenic cyanotoxin microcystin-LR in a novel indigenous bacterium.
- Source :
-
Water Research . May2020, Vol. 174, pN.PAG-N.PAG. 1p. - Publication Year :
- 2020
-
Abstract
- Microcystin-leucine-arginine (MC-LR), a cyclic potentially carcinogenic hepatotoxin, occurs frequently in aquatic habitats worldwide and seriously threatens ecosystem and public health. Limited effectiveness of physicochemical treatments to remove MC-LR from drinking water has led to a search for alternative cost-effective and environment friendly biodegradation strategies. Obtaining MC-degrading bacteria and understanding their MC-degrading mechanisms are outstanding challenges. Here, a novel indigenous bacterium named Sphingopyxis sp. YF1 with a high efficient capacity for MC-degradation was successfully isolated from eutrophic Lake Taihu. Through integrating mass spectrometer and multi-omics analyses accompanied by functional verification of certain genes and proteins, a complete MC-degradation pathway was firstly identified, in which MC-LR was sequentially degraded into linearized MC-LR, tetrapeptide, Adda, phenylacetic acid, and finally potential product CO 2. Some specific proteins such as microcystinase, linearized-microcystinase, tetrapeptidease and PAAase responsible for this pathway were identified. This study pioneeringly demonstrated that MC-LR can be completely degraded through natural remediation processes and revealed a significant potential for MC-LR biodegradation in both natural environment and engineered systems. Image 1 • New MC-degrading genes and products were explored through a multi-omic approach. • A complete MC degradation pathway was identified. • A novel bacterium with high MC-degrading rate was isolated from Lake Taihu. • Proteomics data of MC-degrading bacteria were obtained for the first time. • A series of key enzymes responsible for detoxifying MC-LR were identified. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00431354
- Volume :
- 174
- Database :
- Academic Search Index
- Journal :
- Water Research
- Publication Type :
- Academic Journal
- Accession number :
- 142334865
- Full Text :
- https://doi.org/10.1016/j.watres.2020.115638