Acinetobacter radioresistens strain KA شناسایی و بررسی کارایی باکتر ی بومی 2 جداساز یشده از لج نهای نفتی جهت تجزیه نفت خام

Background and Aim The widespread application of crude oil and its products has caused numerous environmental pollutions. This study aimed to isolate, identify, and determine a bacterial strain’s potential of oil degradation isolated form oily sludge. Methods & Materials After preparing the oily sludge in the sterile containers and cultivating in Bushnell-Haas medium, 24 distinct bacterial colonies were obtained. After performing biochemical and molecular tests, the “Acinetobacter radioresistens strain KA2” with the highest growth rate and crude oil degradation was selected. Then, degradation of various concentrations of crude oil at different PHs (5, 6, 7, 8 and 9), bacterial adherence to hydrocarbons and emulsification index of the selected strain were measured. Total petroleum hydrocarbons were determined by gas chromatography. Ethical Considerations This study has been approved by the Research Ethics Committee of Islamic Azad University of Kermanshah Branch (Code: 19250587962001 ). Results The results indicated that the removal efficiency of crude oil at concentrations of 1, 2, 3, 4, and 5% were 65.24, 76.14, 53.81, 31.84, and 25.21%, respectively. Crude oil removal at pH values of 5, 6, 7, 8, and 9 was 42.4, 69.16, 65.24, 59.41 and 48.24%, respectively. Bacterial adherence to hydrocarbons and emulsification index of the isolated strain were calculated to be 13.69 and 59.14%. Conclusion The isolated bacterium is an efficient strain in treating the crude oil and petroleum compounds. [ABSTRACT FROM AUTHOR]