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Anticancer and antimetastatic activity of copper(II)-tropolone complex against human breast cancer cells, breast multicellular spheroids and mammospheres.
- Source :
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Journal of Inorganic Biochemistry . Mar2020, Vol. 204, pN.PAG-N.PAG. 1p. - Publication Year :
- 2020
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Abstract
- The goal of this work was to display the anticancer and antimetastatic activity of a copper(II) with tropolone (trp), complex [ Cu(trp) 2 ] toward human breast cancer cells in monolayer (2D) and spheroids (3D). Cytotoxicity assays against MCF7 (IC 50(complex) = 5.2 ± 1.8 μM, IC 50(CDDP) = 19.3 ± 2.1 μM) and MDA-MB-231 (IC 50(complex) = 4.0 ± 0.2 μM, IC 50(CDDP) = 27.0 ± 1.9 μM) demonstrate that [ Cu(trp) 2 ] exert greater antitumor potency than cisplatin (CDDP) on 2D and 3D human breast cancer cell models. Besides, [ Cu(trp) 2 ] inhibits cell migration by reducing the metalloproteinases activities and the compound undergoes the breast cancer cells to apoptosis at lower concentrations (2.5–10 μM). Moreover, [ Cu(trp) 2 ] overcame CDDP presenting an IC 50 value 26-fold more lower against breast multicellular spheroids ((IC 50(complex) = 4.9 μM, IC 50(CDDP) = 130 μM)). Also, our results showed that [ Cu(trp) 2 ] inhibited the cell migration and cell invasion of breast multicellular spheroids, showing that [ Cu(trp) 2 ] exhibited antimetastatic properties. On the other hand, [ Cu(trp) 2 ] reduced mammosphere forming capacity affecting the size and number of mammospheres. Taken together, [ Cu(trp) 2 ] exhibited anticancer and antimetastatic properties on monolayer (2D) and spheroids (3D) derived from human breast cancer cells. Copper-tropolone complex impaired cell viability of 2D and 3D breast cancer cell models. Reducing cell migration through inhibiting the metalloproteinases activities. Unlabelled Image • The aim of this work was to show the antitumor actions of a copper(II)-tropolone. • Cytotoxicity studies toward 2D and 3D cell cultured human breast cancer cells. • Complex exerts stronger anticancer activity than cisplatin on breast cancer cell models. • Compound inhibits cell migration through inhibiting the metalloproteinases activities. • Complex reduced mammosphere forming capacity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01620134
- Volume :
- 204
- Database :
- Academic Search Index
- Journal :
- Journal of Inorganic Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 142110115
- Full Text :
- https://doi.org/10.1016/j.jinorgbio.2019.110975