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Q-SNARE protein FgSyn8 plays important role in growth, DON production and pathogenicity of Fusarium graminearum.

Authors :
Adnan, Muhammad
Islam, Waqar
Noman, Ali
Hussain, Ansar
Anwar, Muhammad
Khan, Muhammad Umar
Akram, Waheed
Ashraf, Muhammad Furqan
Raza, Muhammad Fahad
Source :
Microbial Pathogenesis. Mar2020, Vol. 140, pN.PAG-N.PAG. 1p.
Publication Year :
2020

Abstract

SNAREs (Soluble N -ethylmaleimide-sensitive factor attachment protein receptors) help intracellular vesicle trafficking and membrane fusion among eukaryotes. They are vital for growth and development of phyto-pathogenic fungi such as Fusarium graminearum which causes Fusarium Head Blight (FHB) of wheat and barley. The SNARE protein Syn8 and its homologues play many roles among different organisms. Here, we have characterized FgSyn8 in F. graminearum as a homologue of Syn8. We have integrated biochemical, microbiological and molecular genetic approaches to investigate the roles of this protein. Our results reveal that FgSyn8 is indispensable for normal vegetative growth, conidiation, conidial morphology and pathogenicity of F. graminearum. Deoxynivalenol (DON) biochemical assay reveals active participation of this protein in DON production of F. graminearum. This has further been confirmed by the production of bulbous structures among the intercalary hyphae. FgSyn8 mutant strain produced defects in perithecia formation which portrays its role in sexual reproduction. In summary, our results support that the SNARE protein FgSyn8 is required for vegetative growth, sexual reproduction, DON production and pathogenicity of F. graminearum. • SNAREs help intracellular vesicle trafficking and membrane fusion. • The SNARE protein Syn8 play many roles in different organisms. • Here, we have characterized FgSyn8 in F. graminearum. • The results reveal that FgSyn8 is indispensable for different roles of F. graminearum. • Deoxynivalenol (DON) reveals active participation of FgSyn8 in DON production. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
08824010
Volume :
140
Database :
Academic Search Index
Journal :
Microbial Pathogenesis
Publication Type :
Academic Journal
Accession number :
141845976
Full Text :
https://doi.org/10.1016/j.micpath.2019.103948