Development of Twenty-Six Novel Microsatellite Markers in Pampus argenteus (Perciformes: Stromateidae) and Cross-Species Amplification in Closely Related Species, Pampus minor.

Pampus argenteus (Perciformes: Stromateidae) is an economically important benthopelagic fish, which is widely distributed along the coast of China, Indian Ocean, Arabian Gulf and North Sea. To benefit population genetics studies and resource management, 26 pairs of novel microsatellite loci of P. argenteus were isolated using combined biotin capture method. These loci all included di-nucleotide repeat motifs. All markers showed polymorphism when assessed in 30 individuals from Xiapu population. The number of alleles per locus (NA) ranged from 4 to 22, and polymorphism information content (PICs) were from 0.565 to 0.924. Mean values of observed (HO) and expected (HE) heterozygosities ranged from 0.267 to 1.000 and from 0.633 to 0.929, respectively. Cross-species amplification showed that 22 microsatellite markers were successfully amplified in 30 individuals of P. minor, a closely related species of P. argenteus, and indicated clearly polymorphisms. The markers developed in this study will provide a useful tool for investigating the genetic structure, gene flow, and mating system of P. argenteus and the closely related species. They will also facilitate study on their evolutionary responses to the ongoing coastal systems change. [ABSTRACT FROM AUTHOR]