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Translation of two-photon microscopy to the clinic: multimodal multiphoton CARS tomography of in vivo human skin.
- Source :
-
Journal of Biomedical Optics . Jan2020, Vol. 25 Issue 1, p14515-14515. 1p. - Publication Year :
- 2020
-
Abstract
- Two-photon microscopes have been successfully translated into clinical imaging tools to obtain high-resolution optical biopsies for in vivo histology. We report on clinical multiphoton coherent anti-Stokes Raman spectroscopy (CARS) tomography based on two tunable ultrashort near-infrared laser beams for label-free in vivo multimodal skin imaging. The multiphoton biopsies were obtained with the compact tomograph "MPTflex-CARS" using a photonic crystal fiber, an optomechanical articulated arm, and a four-detector-360 deg measurement head. The multiphoton tomograph has been employed to patients in a hospital with diseased skin. The clinical study involved 16 subjects, 8 patients with atopic dermatitis, 4 patients with psoriasis vulgaris, and 4 volunteers served as control. Two-photon cellular autofluorescence lifetime, second harmonic generation (SHG) of collagen, and CARS of intratissue lipids/proteins have been detected with single-photon sensitivity, submicron spatial resolution, and picosecond temporal resolution. The most important signal was the autofluorescence from nicotinamide adenine dinucleotide [NAD(P)H]. The SHG signal from collagen was mainly used to detect the epidermal–dermal junction and to calculate the ratio elastin/collagen. The CARS/Raman signal provided add-on information. Based on this view on the disease-affected skin on a subcellular level, skin areas affected by dermatitis and by psoriasis could be clearly identified. Multimodal multiphoton tomographs may become important label-free clinical high-resolution imaging tools for in vivo skin histology to realize rapid early diagnosis as well as treatment control. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10833668
- Volume :
- 25
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Biomedical Optics
- Publication Type :
- Academic Journal
- Accession number :
- 141566324
- Full Text :
- https://doi.org/10.1117/1.JBO.25.1.014515