火龙果成年植株茎段直接诱导不定芽快繁体系建立.

[Objective] This paper aimed to establish an efficient pitaya fruit rapid propagation system and preserve the superior traits of the female parent. [Method] Hie stem segments of adult pitaya plant were used as explants to study the effects of different growth regulators and ratios, basic medium concentration and explant type on the induction of adventitious buds in stems. And thereby screen the best culture conditions suitable for adventitious buds proliferation, rooting and transplanting. [Result] The explants with splinters and nap on the stem segments of pitaya had the greatest adventitious bud induction rate (85.1 % ) , which was significantly greater than the explants with splinters but no thorns and fluff. The induction rate of adventitious buds of explants with no splinters was 0; The medium suitable for adventitious bud induction of pitaya was MS + 6-BA 2. 0 mg/L + NAA 0.1 -0.2 mg/L; the medium with the greatest adventitious bud proliferation coefficient was MS + 6-BA 0. 5 mg/L + NAA 0. 2 mg/L + Tryptone 5(X) mg/L; Suitable medium for adventitious bud rooting was 6-BA 1.0 mg/L + NAA 0.1 -0.2 mg/L + CCC 0.5 - 1.0 mg/ L + AC 0. 03 % - 0. 05 % , the rooting rate was above 98 % ; The substrate with high survival rate and good growth rate was perlite + humus soil (2:1) or vermiculite + humus soil (1:1). [ABSTRACT FROM AUTHOR]