Back to Search Start Over

Cell release during perfusion reflects cold ischemic injury in rat livers.

Authors :
de Vries, Reinier J.
Pendexter, Casie A.
Cronin, Stephanie E. J.
Marques, Beatriz
Hafiz, Ehab O. A.
Muzikansky, Alona
van Gulik, Thomas M.
Markmann, James F.
Stott, Shannon L.
Yeh, Heidi
Toner, Mehmet
Uygun, Korkut
Tessier, Shannon N.
Source :
Scientific Reports. 1/24/2020, Vol. 10 Issue 1, p1-14. 14p.
Publication Year :
2020

Abstract

The global shortage of donor organs has made it crucial to deeply understand and better predict donor liver viability. However, biomarkers that effectively assess viability of marginal grafts for organ transplantation are currently lacking. Here, we showed that hepatocytes, sinusoidal endothelial, stellate, and liver-specific immune cells were released into perfusates from Lewis rat livers as a result of cold ischemia and machine perfusion. Perfusate comparison analysis of fresh livers and cold ischemic livers showed that the released cell profiles were significantly altered by the duration of cold ischemia. Our findings show for the first time that parenchymal cells are released from organs under non-proliferative pathological conditions, correlating with the degree of ischemic injury. Thus, perfusate cell profiles could serve as potential biomarkers of graft viability and indicators of specific injury mechanisms during organ handling and transplantation. Further, parenchymal cell release may have applications in other pathological conditions beyond organ transplantation. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20452322
Volume :
10
Issue :
1
Database :
Academic Search Index
Journal :
Scientific Reports
Publication Type :
Academic Journal
Accession number :
141385261
Full Text :
https://doi.org/10.1038/s41598-020-57589-4