Enhanced bio-hydrogen production from cornstalk hydrolysate pretreated by alkaline-enzymolysis with orthogonal design method.

Bio-hydrogen (H 2) production from renewable biomass has been accepted as a promising method to produce an alternative fuel for the future. In this study, fermentative hydrogen production from cornstalk (CS) hydrolysate pretreated by alkaline-enzymolysis method was investigated. Meanwhile, a five-factor and five-level orthogonal experimental array was designed to study the influences of Ca(OH) 2 concentration, alkaline hydrolysis time, alkaline hydrolysis temperature, cellulase and xylanase dosages on cornstalk pretreatment and hydrogen production. A maximum reducing sugar yield of 0.59 g/g-CS was obtained at Ca(OH) 2 0.5%, hydrolysis temperature 115 °C, hydrolysis time 1.5 h, cellulase dosage 4000 U/g-CS and xylanase 4000 U/g-CS. Under this same condition, the maximum hydrogen yields of 168.9 mL/g-CS, 357.6 mL/g-CS, and 424.3 mL/g-CS were obtained at dark-fermentation, photo-fermentation, and two-stage fermentation respectively. It's also found that the significance of these five parameters on H 2 production followed from high to low order as: Ca(OH) 2 concentration, cellulase dosage, xylanase dosage, hydrolysis time, and hydrolysis temperature. By comparing the energy produced with the energy spent, the maximum Energy Sustainability Index (ESI) value of 1.11 was obtained at the two-stage fermentation. The results suggested that two-stage fermentation is a promising and efficient way for hydrogen production from lignocellulosic biomass. • Cornstalk pretreated by Ca(OH) 2 -enzymolysis was used for bio-H 2 production. • Parameters of cornstalk pretreatment were optimized by a 55 orthogonal design. • A maximum H 2 yield of 424.3 mL/g-cornstalk was obtained at two-stage fermentation. • A maximum ESI value of 1.11 was obtained at two-stage fermentation. • Two-stage fermentation is a promising way to produce H 2 from agricultural biomass. [ABSTRACT FROM AUTHOR]