FOXM1-activated LINC01094 promotes clear cell renal cell carcinoma development via miR-224-5p/CHSY1.

Clear cell renal cell carcinoma (ccRCC) is regarded as the most aggressive subtype of RCC with high rates of metastasis and recurrence. An extensive body of studies had proved long non-coding RNAs lncRNAs) play pivotal parts in the development and evolution of diverse malignant tumors. However, the potential of LINC01094 in ccRCC tumorigenesis is still unexplored. In the present research, with the aid of TCGA database, we found LINC01094 was high-expressed in ccRCC tissues. Upregulation of LINC01094 was also confirmed in ccRCC cell lines and functional experiments delineated that LINC01094 knockdown led to inhibition on ccRCC cell growth and metastasis. Moreover, LINC01094 was activated by FOXM1 at the transcriptional level. Further assay demonstrated that LINC01094 worked as a sponge of miR-224-5p and CHSY1 was a miR-224-5p-targeted mRNA. Furtherly, we verified that LINC01094 acted as a ceRNA in ccRCC to regulate CHSY1 expression via competitively bind to miR-224-5p. Lastly, our results expounded that LINC01094 exerted its tumor-promoting performance in ccRCC development through miR-224-5p/CHSY1 regulatory axis, which shed light on the molecular mechanism underlying LINC01094 in ccRCC and opened a new prospective for the treatment of ccRCC. [ABSTRACT FROM AUTHOR]