Back to Search
Start Over
Expression of the bovine KLF6 gene polymorphisms and their association with carcass and body measures in Qinchuan cattle (Bos Taurus).
- Source :
-
Genomics . Jan2020, Vol. 112 Issue 1, p423-431. 9p. - Publication Year :
- 2020
-
Abstract
- Kruppel-like factor 6 (KLF6) genes plays a significant role in the regulation of cell differentiation, proliferation and muscle development. The aim of this study is to investigate the genetic variation and the haplotype combination of the KLF6 gene in Qinchuan cattle and verify its contribution to bovine carcass traits and body measurements. The data were analyzed by real-time quantitative PCR (qPCR) to detect the expression profile of the KLF6 gene in the various tissues of Qinchuan cattle. PCR amplicons sequencing explored three novel SNPs at loci 3332C > G; 3413C > T and 3521G > A in the 2nd exon region of the KLF6 gene. The expression of KLF6 in the liver, kidney and lung was greater than that of other tissues. Allelic and genotypic frequencies of these SNPs were found to be in Hardy Weinberg equilibrium (P < 0.05). In SNP1, genotype CC, in SNP2, genotype CT and in SNP3 genotype GG were associated (P < 0.05) with larger body and carcass measurements. Association analysis results indicated that individuals with the Hap1/4 diplotype had a longer body and rump, were taller at the withers, and were wider at the hip than the other combinations. In terms of ultrasound carcass measures, Hap1/4 was associated with a larger muscle area and more intramuscular fat than other combinations. The bioinformatics study of the KLF6 protein showed a high degree of conservation in different mammalian species. The above results suggest that the KLF6 gene can used as potential candidate markers gene for the beef breed improvement through marker assisted selection of Qinchuan cattle. • Spatiotemporal expression of KLF6 was identified in different tissues within two age groups of Qinchuan cattle. • Bioinformatics study was conducted for the estimates of conservation and biological evolution of KLF6 gene. • The haplotype combination 1/4 could be consider a suitable marker in MAS for breed improvement program. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08887543
- Volume :
- 112
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 141171223
- Full Text :
- https://doi.org/10.1016/j.ygeno.2019.03.005