Enzymatic method and its validation for the micromolar assay of glucose in human serum samples.

This work depicts the novel chromogenic system for the assay of glucose in blood samples connected with the chelate formation of sulfosalicylic acid (SSA) with iron (Fe(III)) using glucose oxidase (GOD) presented. The purple-colored Fe-SSA chelate compound formed, maintain a strong absorption at 500 nm in the acidic buffer of pH 3.8. The Beer's law limit for the assay of glucose by rate, and a one-time method is in the range of 46–1295 μmol/L and 9–1110 μmol/L sequentially. Inter and Intraday precision fluctuated amid 0.98–1.4% (n = 10), and 1.33–2.89% (n = 15) sequentially. The recovery of glucose varied from 96.6 to 102%, registering trifling interference by common interferants in blood samples. The accuracy of the outcome, LOD, and LOQ of glucose were within 90–102%, 2.376, and 7.923 μmol/L individually. The introduced method has a coefficient of correlation 0.999 with the standard kit system, and easy to ascertain serum glucose with excellent recovery and insignificant interruption. Nevertheless, the system can be recognized for selection by the biochemical laboratories. Image 1 • The enzymatic pathway is projected for quantification of the clinically significant biomarker. • The proposed method demands the use of a single enzyme for the assay of glucose. • Sulfosalicylic acid is used as a chromogenic probe. • The colored product formed has a high molar extinction coefficient and gives reliable and highly précised results. • The effectiveness of the method has been proved by evaluating kinetic parameters. [ABSTRACT FROM AUTHOR]