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Deficient Active Transport Activity in Healing Mucosa After Mild Gastric Epithelial Damage.
- Source :
-
Digestive Diseases & Sciences . Jan2020, Vol. 65 Issue 1, p119-131. 13p. - Publication Year :
- 2020
-
Abstract
- <bold>Background: </bold>Peptic ulcers recur, suggesting that ulcer healing may leave tissue predisposed to subsequent damage. In mice, we have identified that the regenerated epithelium found after ulcer healing will remain abnormal for months after healing.<bold>Aim: </bold>To determine whether healed gastric mucosa has altered epithelial function, as measured by electrophysiologic parameters.<bold>Method: </bold>Ulcers were induced in mouse gastric corpus by serosal local application of acetic acid. Thirty days or 8 months after ulcer induction, tissue was mounted in an Ussing chamber. Transepithelial electrophysiologic parameters (short-circuit current, Isc. resistance, R) were compared between the regenerated healed ulcer region and the non-ulcerated contralateral region, in response to luminal hyperosmolar NaCl challenge (0.5 M).<bold>Results: </bold>In unperturbed stomach, luminal application of hyperosmolar NaCl transiently dropped Isc followed by gradual recovery over 2 h. Compared to the starting baseline Isc, percent Isc recovery was reduced in 30-day healing mucosa, but not at 8 months. Prior to NaCl challenge, a lower baseline Isc was observed in trefoil factor 2 (TFF2) knockout (KO) versus wild type (WT), with no Isc recovery in either non-ulcerated or healing mucosa of KO. Inhibiting Na/H exchanger (NHE) transport in WT mucosa inhibited Isc recovery in response to luminal challenge. NHE2-KO baseline Isc was reduced versus NHE2-WT. In murine gastric organoids, NHE inhibition slowed recovery of intracellular pH and delayed the repair of photic induced damage.<bold>Conclusion: </bold>Healing gastric mucosa has deficient electrophysiological recovery in response to hypertonic NaCl. TFF2 and NHE2 contribute to Isc regulation, and the recovery and healing of transepithelial function. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BIOLOGICAL transport
*MUCOUS membranes
*GASTRIC mucosa
*PEPTIC ulcer
*SHORT-circuit currents
*SALT
*WOUND healing
*BIOLOGICAL models
*RESEARCH
*HYDROGEN-ion concentration
*HYPERTONIC solutions
*ANIMAL experimentation
*TIME
*RESEARCH methodology
*EVALUATION research
*MEDICAL cooperation
*COMPARATIVE studies
*ACETIC acid
*BIOELECTRIC impedance
*RESEARCH funding
*EPITHELIAL cells
*MICE
Subjects
Details
- Language :
- English
- ISSN :
- 01632116
- Volume :
- 65
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Digestive Diseases & Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 141077939
- Full Text :
- https://doi.org/10.1007/s10620-019-05825-x