胃内容物中甲卡西酮LC-MS/MS检验方法研究.

Objective: To establish a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of methcathinone and its metabolite cathinone in gastric contents. Methods: Proadifen hydrochloride (SKF525A) was used as internal standard. Column Eclipse Plus C18 column 1.8 μm, 100 mm× 3 mm was used as stationary phase. Mobile phase was 0.1 % formic acid aqueous solution and acetonitrile, gradient elution with flow rate of 0.3 mL/min and injection volume of 1 μL. Electrospray ion source (ESI) was detected by positive ion multi reaction monitoring mode MRM. Results: The linear range of methcathinone in gastric contents was 1-5000 ng/g, and the standard curve equation was Y=0.026X+0.4501 R²=0.9998, the detection limit was 0.5 ng/g, and the quantitative limit was 1.0 ng/g. The linear range of cathinone in gastric contents was 2-5000 ng/g, and the standard curve equation was Y=0.0099X+0.1576 R²=0.9997, the detection limit was 1.0 ng/g, and the quantitative limit was 2.0 ng/g. Conclusion: The established method for the detection of methcathinone and its metabolite cathinone in the gastric contents is simple and sensitive. This method can be used for the detection of methcathinone poisoning in the public security. [ABSTRACT FROM AUTHOR]