淫羊藿苷对兔股骨MEG3、H19和DANCR表达的影响.

Objective To study the effects of icariin on the expression of long non-coding RNA MEG3(maternally expressed gene 3), H19, DANCR/ANCR(anti-differentiation ncRNA) and BMP2 in different parts of the bone of New Zealand white rabbits after ovariectomy. Methods 48 New Zealand white rabbits(age of 7 months) were used to make the model of osteoporosis group, which was randomly divided into 3 groups as sham operation Group, ovariectomized model group(OVX) and icariin treatment Group(OVX plus treatment) according to body weight. From the 4 th week after operation, the treatment group received gastric administration of icariin [20 mg/(kg·d)], and the sham and OVX groups received the same amount of saline every day for 8 weeks. Three-dimensional reconstruction of μCT images was performed 12 weeks after operation to evaluate the establishment of osteoporosis model and femur BV/TV, Tb·Th, Tb·N BS/BV, Tb·Sp and other changes. The expression level of long chain non-encoded RNA MEG3, H19 and DANCR and the mRNA expression level of Runx2 in femur, vertebrae and skull tissue and were detected by qRT-PCR at 12 weeks after operation. Immunohistochemistry was used to detect the expression of BMP2 in femoral paraffin section. Results Compared with the ovariectomized model group, icariin reduced the level of long-chain non-coding RNA MEG3, H19 and DANCR to improve the volume of femoral trabeculae in osteoporosis rabbits, while the effect of icariin on the three lncRNAs in the vertebrae was mainly on reducing the expression level of MEG3(P<0.05), and the influence on the skull was mainly on the changes in DANCR expression. The expression of BMP2 in femur treated by icariin was the highest. Conclusion Studying the effect of icariin on lncRNA has guiding significance for the treatment of osteoporosis. [ABSTRACT FROM AUTHOR]