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Engineering the oleaginous yeast Yarrowia lipolytica for production of α-farnesene.
- Source :
-
Biotechnology for Biofuels . 12/23/2019, Vol. 12 Issue 1, p1-11. 11p. - Publication Year :
- 2019
-
Abstract
- Background: Yarrowia lipolytica, a non-traditional oil yeast, has been widely used as a platform for lipid production. However, the production of other chemicals such as terpenoids in engineered Y. lipolytica is still low. α-Farnesene, a sesquiterpene, can be used in medicine, bioenergy and other fields, and has very high economic value. Here, we used α-farnesene as an example to explore the potential of Y. lipolytica for terpenoid production. Results: We constructed libraries of strains overexpressing mevalonate pathway and α-farnesene synthase genes by non-homologous end-joining (NHEJ) mediated integration into the Y. lipolytica chromosome. First, a mevalonate overproduction strain was selected by overexpressing relevant genes and changing the cofactor specificity. Based on this strain, the downstream α-farnesene synthesis pathway was overexpressed by iterative integration. Culture conditions were also optimized. A strain that produced 25.55 g/L α-farnesene was obtained. This is the highest terpenoid titer reported in Y. lipolytica. Conclusions: Yarrowia lipolytica is a potentially valuable species for terpenoid production, and NHEJ-mediated modular integration is effective for expression library construction and screening of high-producer strains. [ABSTRACT FROM AUTHOR]
- Subjects :
- *YEAST
*CHROMOSOMES
*OVERPRODUCTION
*TITERS
*LIPIDS
Subjects
Details
- Language :
- English
- ISSN :
- 17546834
- Volume :
- 12
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Biotechnology for Biofuels
- Publication Type :
- Academic Journal
- Accession number :
- 140850465
- Full Text :
- https://doi.org/10.1186/s13068-019-1636-z