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Bioactive Polyphenols Modulate Enzymes Involved in Grapevine Pathogenesis and Chitinase Activity at Increasing Complexity Levels.

Authors :
Filippi, Antonio
Petrussa, Elisa
Boscutti, Francesco
Vuerich, Marco
Vrhovsek, Urska
Rabiei, Zohreh
Braidot, Enrico
Source :
International Journal of Molecular Sciences. Dec2019, Vol. 20 Issue 24, p6357. 1p.
Publication Year :
2019

Abstract

The reduction of synthetic chemistry use in modern viticulture relies on either the biological control of microorganisms or the induction of pathogenesis-related proteins. In the present study, the effects of hydro-alcoholic plant extracts (PEs) (i.e., by-products of Vitis vinifera L., leaves of Olea europaea L. and Ailanthus altissima (Mill.) Swingle) were tested on purified enzymes activity involved in plant-pathogen interactions. The polyphenolic composition was assayed and analyzed to characterize the extract profiles. In addition, suspension cell cultures of grapevine were treated with PEs to study their modulation of chitinase activity. Application of grape marc's PE enhanced chitinase activity at 4 g L−1. Additionally, foliar treatment of grape marc's PE at two doses (4 g L−1 and 800 g L−1) on grapevine cuttings induced a concentration-dependent stimulation of chitinase activity. The obtained results showed that the application of bioactive compounds based on PEs, rich in phenolic compounds, was effective both at in vitro and ex/in vivo level. The overall effects of PEs on plant-pathogen interaction were further discussed by applying a multi-criteria decision analysis, showing that grape marc was the most effective extract. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
16616596
Volume :
20
Issue :
24
Database :
Academic Search Index
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
140824635
Full Text :
https://doi.org/10.3390/ijms20246357