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Human carriage of cefotaxime-resistant Escherichia coli in North-East India: an analysis of STs and associated resistance mechanisms.
- Source :
-
Journal of Antimicrobial Chemotherapy (JAC) . Jan2020, Vol. 75 Issue 1, p72-76. 5p. - Publication Year :
- 2020
-
Abstract
- <bold>Objectives: </bold>To determine the prevalence of Escherichia coli STs and associated resistance mechanisms carried by the community in North-East India.<bold>Methods: </bold>E. coli (108) were isolated from sewage collected from 19 sites across the city of Silchar by plating on MacConkey agar with/without selection (50 mg/L cefotaxime). Species identification was confirmed by MALDI-TOF MS for 82 isolates. Common resistance mechanisms were determined by WGS of pooled E. coli isolates. PFGE combined with specific probes determined the presence of common resistance mechanisms in all isolates. Phylotypes, multilocus STs, core-genome multilocus STs, resistance genes and virulence genes were determined by in silico analysis of 38 genomes.<bold>Results and Conclusions: </bold>Analysis of isolates collected without selection (n=33) indicated that cefotaxime resistance in E. coli was 42% (14/33) and estimated meropenem resistance at 9%. The remaining 58% (19/33) were additionally susceptible to ampicillin, trimethoprim, ciprofloxacin and aminoglycosides. The most common ST among the cefotaxime-resistant E. coli was ST167 (29%), followed by ST410 (17%) and ST648 (10%). E. coli ST131 was absent from the collection. Sixty-three isolates were resistant to cefotaxime and harboured blaCTX-M-15 [54% (34/63)] or blaCMY-42 [46% (29/63)], of which 10% (6/63) harboured both genes. Carbapenem resistance was due to blaNDM-5, found in 10/63 cefotaxime-resistant isolates, and/or blaOXA-181, found in 4/63 isolates. NDM-5 was encoded by IncX3 and/or IncFII plasmids and CMY-42 was mostly encoded by IncI plasmids. NDM-5 appears to have replaced NDM-1 in this region and CMY-42 appears to be in the process of replacing CTX-M-15. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CARBAPENEMS
*MATRIX-assisted laser desorption-ionization
*PLASMIDS
*ESCHERICHIA coli
*BACTERIAL proteins
*RESEARCH
*PULSED-field gel electrophoresis
*SEQUENCE analysis
*BIOLOGICAL evolution
*RESEARCH methodology
*CEFOTAXIME
*MEDICAL cooperation
*EVALUATION research
*HYDROLASES
*COMPARATIVE studies
*GENOMES
*ESCHERICHIA coli diseases
*DISEASE prevalence
*CARRIER state (Communicable diseases)
*DRUG resistance in microorganisms
*SEWAGE
*ANTIBIOTICS
*MICROBIAL sensitivity tests
*PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 03057453
- Volume :
- 75
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Antimicrobial Chemotherapy (JAC)
- Publication Type :
- Academic Journal
- Accession number :
- 140358329
- Full Text :
- https://doi.org/10.1093/jac/dkz416