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Simultaneous comparison of L-NAME and melatonin effects on RAW 264.7 cell line's iNOS production and activity.
- Source :
-
Turkish Journal of Biochemistry / Turk Biyokimya Dergisi . Dec2019, Vol. 44 Issue 6, p840-847. 8p. - Publication Year :
- 2019
-
Abstract
- Background: NO (nitric oxide) inhibition could be used for evaluating the drug's efficacy for NO-mediated inflammatory disorders. The aim of this study was to investigate the influence of L-NAME and melatonin on different NO production levels in RAW 264.7 cell line as an in vitro model for inflammatory diseases. Materials and methods: RAW 264.7 macrophage cell line was used to compare the effects of L-NAME and melatonin on basal and Lipopolysaccharide (LPS)-induced iNOS levels. The cells were treated using L-NAME and melatonin for 1 h, afterward incubated with/without LPS for 8 and 24 h. Finally, iNOS mRNA, protein, activity, and nitrite concentrations were evaluated. Results: Inhibition rate of nitrite by 1 mM L-NAME compared with LPS control were 78% and 80% during 8 and 24 h, respectively. Real-time PCR showed that in the LPStreated group, 1 mM L-NAME could result in 14% increase of iNOS-mRNA compared with the control group during 8 h. Dose-dependent activity of iNOS in LPS-induced cells from non-treated to 4 mM L-NAME showed 79% reduction while at the same concentrations of melatonin this decrease was 32% (p-value <0.05). Conclusion: L-NAME showed lower iNOS expression modulating efficacy than melatonin. The result concluded lower potential of the NOS synthetic inhibitors rather than melatonin in the treatment of NO-related disorders. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CELL lines
*MELATONIN
*DRUG efficacy
*NITRIC oxide
Subjects
Details
- Language :
- English
- ISSN :
- 02504685
- Volume :
- 44
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Turkish Journal of Biochemistry / Turk Biyokimya Dergisi
- Publication Type :
- Academic Journal
- Accession number :
- 140346061
- Full Text :
- https://doi.org/10.1515/tjb-2019-0209