Back to Search
Start Over
Effects of normothermic microwave irradiation on CD44+/CD24‒ in breast cancer MDA-MB-231 and MCF-7 cell lines.
- Source :
-
Bioscience, Biotechnology & Biochemistry . Jan2020, Vol. 84 Issue 1, p103-110. 8p. - Publication Year :
- 2020
-
Abstract
- We previously reported that MDA-MB-231 and MCF-7 cells, which are breast cancer cell lines and have cancer and cancer-initiating cells (CICs), were killed following normothermic microwave irradiation in which the cellular temperature was maintained at 37°C. In this study, we investigated the percentages of live or dead cells among CD44+/CD24− cells, which were defined as CICs among MDA-MB-231 and MCF-7 cells, and other types of cells in response to microwave irradiation. CD44+/CD24− cells among MDA-MB-231 cells were killed, thereby decreasing the number of cells, whereas the number of live CD44+/CD24− MCF-7 cells was increased following microwave irradiation. Moreover, adhesion, invasion, and migration were decreased in MDA-MB-231 cells, and the activation of matrix metalloproteinase-2 (MMP-2) in MDA-MB-231 cells was increased following microwave irradiation. These decreased cell activities might have been caused by MMP-2 activation and population changes in CD44+/CD24− in MDA-MB-231 cells. Abbreviations: APC: allophecocyanin; CBB: coomassie Brilliant Blue; CD: cluster of differentiation; CICs: cancer-initiating cells; FACS: fluorescence-activated cell sorting; FBS: fetal bovine serum; FITC: fluorescein isothiocyanate; FTDT: finite-difference time domain; HER2: human epidermal growth factor receptor type 2; PI: propidium iodide. Functional changes of MDA-MB-231 cells by normothermic microwave irradiation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09168451
- Volume :
- 84
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Bioscience, Biotechnology & Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 140088104
- Full Text :
- https://doi.org/10.1080/09168451.2019.1670044