半枝莲和白花蛇舌草药对中总黄酮的提取及纯化工艺研究.

Objective To screen out the optimal conditions for extraction and purification of total flavonoids from the herb pair of Scutellariae barbatae and Hedyotis diffusa. Methods Single factor and L9 (34) orthogonal experiment was de signed with the extraction ratio and extract quantity of totfl flavonoids as indexes,using matching of herb pair,ethanol concentration, solid-liquid ratio, extraction time as inspected elements. The technical process for purification of total flavonoids with the static and dynamic adsorption experiments was screened by adsorption and desorption ratio as targets. Results The optimll process conditions were : ethanol concentration 70%，the matching of herb pair of 4 a 1，the solid-liquid ratio of 1 : 25，extraction for 3 times and 2h for each time. The parameters of the purification process were as follows : concentration of sample 63. 14 mg/ ml and pH value 4. 0, which used in the chromatographic column of polyamide resin at a flow velocity of 1. 5 ml/min. The chromatographic column was eluted with purified water of 3. lBV,and then eluted with 65% ethanol concentration of 9. 3BV at a flow velocity of 3ml/min. The purity of the total flavonoids was 40. 39% in the fine product, and the transfer rate was 8.. 57%. Conclusion The optimal process conditions were reasonable and feasibl.. The purification technique of polyamide resin was uncomplicated and had a good effect. [ABSTRACT FROM AUTHOR]