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The effect of the sub-minimal inhibitory concentration and the concentrations within resistant mutation window of ciprofloxacin on MIC, swimming motility and biofilm formation of Pseudomonas aeruginosa.
- Source :
-
Microbial Pathogenesis . Dec2019, Vol. 137, pN.PAG-N.PAG. 1p. - Publication Year :
- 2019
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Abstract
- To explore the effect of sub-minimal inhibitory concentration (sub-MIC) and concentrations within resistant mutation window (MSW) of ciprofloxacin (CIP) on minimal inhibitory concentration (MIC), swimming motility and biofilm formation of Pseudomonas aeruginosa , and also to investigate the correlation between swimming motility and genes expression of lasI, lasR , rhlI, rhlR and pqsR. The collected strains were incubated under four different concentrations for 5 days. The MIC and mutant prevention concentration (MPC) were measured by the agar dilution method. The diameter of turbid cycle was used to signify the swimming motility. The biofilm formation was measured by the crystal violet stain method. The genes expression of lasI , lasR , rhlI , rhlR and pqsR were measured by RT-PCR. A total of 11 P. aeruginosa which sensitive to CIP were collected. The incubation within concentrations of MSW made MICs to CIP increased more obviously than under sub-MIC (P < 0.05). The swimming motility showed a trend of being inhibited first and then promoted over time under sub-MIC (P < 0.05), whereas, it was promoted under concentrations within MSW. The biofilm formation was significantly promoted under the concentration of 4×MIC (P < 0.05). Under sub-MIC, the genes expression of rhlR and pqsR had a middle level positive correlation with the promotion of the swimming motility (P < 0.05, r = 0.788 and P < 0.05, r = 0.652, respectively). Under the concentration of sub-MIC (0.5×MIC) and the concentrations within MSW (1×MIC, 2×MIC and 4×MIC), the effect of CIP on MICs, swimming motility and biofilm formation of P.aeruginosa was quite different. The genes expression of rhlR and pqsR had a middle level positive correlation with the promotion of the swimming motility. • The method of this paper was special. We incubated P.aeruginosa with different concentrations for 5 days. We found the variation trend of measurements over time and differences in the effects of different concentrations on the measurements. • In this study, the MICs, resistance rates and ability of biofilm formation and of P.aeruginosa against CIP was higher and stronger at the concentrations within MSW than at the sub-MIC. However, the swimming motility was stronger under the sub-MIC than the concentrations within MSW.The results of our study could be of great value in the establishment of reasonable dosing regimens to prevent resistance of P. aeruginosa to CIP. • Special and rigorous statistical method, repeated analysis of variance, made our conclusions more credible. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08824010
- Volume :
- 137
- Database :
- Academic Search Index
- Journal :
- Microbial Pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 139844715
- Full Text :
- https://doi.org/10.1016/j.micpath.2019.103765