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Bacterias promotoras del crecimiento en plantas con potencial agente biocontrolador a Fusarium oxysporum f . sp. Lycopersici, y Moniliophthora roreri.

Authors :
Javier Guato-Molina, Jefferson
Andrés Auhing-Arcos, Javier
Abel Crespo-Ávila, Jorge
Alejandro Esmeraldas-García, Gabriel
Francisco Mendoza-León, Antonio
Fabricio Canchignia-Martínez, Hayron
Source :
Scientia Agropecuaria. jul-sep2019, Vol. 10 Issue 3, p393-402. 10p.
Publication Year :
2019

Abstract

The objective was to characterize the potential PGPR antagonist in the inhibition of mycelial development, conidia in F. oxysporum and spores in M. roreri . The PCR technique was used for the identification of F. oxysporum and M. roreri . Selecting the bacteria: Acinetobacter calcoaceticus BMR2-12, Klebsiella variicola BO3-4, Enterobacter asburiae BA4-19, Enterobacter asburiae PM3-14, Pseudomonas protegens CHA0, Pseudomonas veronii R4 and Bacillus subtilis ATCC-5540. Antagonistic activities were evaluated: a) Mycelial inhibition in F. oxysporum and M. roreri ; b) Reduction in production of conidia and spores for 12 and 15 days' post-incubation. The PCR confirmed the 670 bp amplification for F. oxysporum. The 750 bp sequencing defined a degree of similarity of 99% to M. roreri , when compared to the NCBI GenBank. The application of B. subtilis is of greater antagonistic activity to inhibition (mycelial and conidia) with (95 and 90%) to F. oxyporum. The activity of BO3-4 in M. roreri totally inhibits mycelial development and spore production 12 and 15 days post - incubation respectively. The selection of these biocontrollers and their application as a consortium will offer an alternative to benefit in the reduction of agrochemicals in the cultivation of tomato and cocoa. [ABSTRACT FROM AUTHOR]

Details

Language :
Spanish
ISSN :
20779917
Volume :
10
Issue :
3
Database :
Academic Search Index
Journal :
Scientia Agropecuaria
Publication Type :
Academic Journal
Accession number :
139638576
Full Text :
https://doi.org/10.17268/sci.agropecu.2019.03.10