Yeast diversity investigation of Vitis davidii Föex during spontaneous fermentations using culture-dependent and high-throughput sequencing approaches.

Vitis davidii Föex is widely planted in South China as a potential wine making grape. In this study, yeast communities during the spontaneous fermentations of two varieties of Vitis davidii Föex in Guizhou, China were investigated. Hanseniaspora uvarum , Pichia terricola , Saccharomyces cerevisiae / S. mikatae , and Schizosaccharomyces japonicus were the four main yeast species detected by culture-dependent and high-throughput sequencing approaches. However, the composition of minor yeast species was quite different as revealed by the two approaches. Ten yeast species including Debaryomyces hansenii , Rhodotorula mucilaginosa , Sporidiobolus paraoseus , Starmerella bacillaris , Zygoascus meyerae , etc. were detected by culture-dependent approaches, whereas the other five species were found by high-throughput sequencing analysis. S. japonicus was widely found during spontaneous fermentations and its concentrations were mostly higher than that of S. cerevisiae. The difference in grape varieties and fermentation conditions contributed to yeast diversity. The results of this study provide basic information on indigenous yeast diversity from Vitis davidii Föex in Guizhou, which would help to exploit the potential of non- Saccharomyces yeast species with good oenological attributes. Unlabelled Image • Vitis davidii Föex showed distinctive yeast diversity in spontaneous fermentations. • Schizosaccharomyces japonicus were predominant yeast species. • Saccharomyces cerevisiae / S. mikatae seemed not be able to compete with S. japonicus. • ITS-amplicon high-throughput sequencing could not detect all yeast species found. [ABSTRACT FROM AUTHOR]