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Structural analysis of the asparagine-linked oligosaccharides of rat haptoglobin metaholically labeled in a hepatocyte culture system.
- Source :
-
European Journal of Biochemistry . 12/1/86, Vol. 161 Issue 2, p315-320. 6p. - Publication Year :
- 1986
-
Abstract
- We analyzed the asparagine-linked oligosaccharide chains of rat haptoglobin which were synthesized and secreted by hepatocytes in primary culture. When the cells were incubated with either [³H]mannose,[³H]galactose, or [³H]fucose, all the radioactive precursors were incorporated into the β subunit of haptoglobin. [³]HMannose-labled haptoglobin was purified from the culture medium by immunoaffinity chromatogrpahy, and [³H]oligosaccharides were prepared by strong alkali-borohydride treatment. The oligosccharides obtained were analyzed by anion-exchange high-performance liquid chromatography, concanavali-A - Sepharose chromatography and Bio-Gel P-4 chromatography before and after sequential exoglycosidase digestions. The oligosaccharides labeled with [³H]fucose or [³H]galactose were also characterized by the above methods. The results indicated that rat haptoglobin contains two complex-type oligosccharide chains in each β subunit one with a possible structure of (± NeuAc→Galβ→GlcNAcβ→)3(Manα→)2Manβ→GlcNAc→(±Fucα→)GcNAc and the other with (±NeuAc→Galβ→GlcNacβ→Manα→)2Manβ→GlcNAc→(±Fucα→)GlcNAc. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 161
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13946759
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1986.tb10449.x