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Marker-assisted backcrossing to improve seed oleic acid content in four elite and popular peanut (Arachis hypogaea L.) cultivars with high oil content.
- Source :
-
Breeding Science . Jun2019, Vol. 69 Issue 2, p234-243. 10p. - Publication Year :
- 2019
-
Abstract
- High oleic acid composition is an important determinant of seed quality in peanut (Arachis hypogaea) in regard to its nutritional benefits for human health and prolonged shelf-life for peanut products. To improve the oleic acid content of popular peanut cultivars in China, four peanut cultivars of different market types were hybridized with high-oleic-acid donors and backcrossed for four generations as recurrent parents using fad2 marker-assisted backcross selection. Seed quality traits in advanced generations derived by selling w'ere assessed using near-infrared reflectance spectroscopy for detection of oleic acid and Kompetitive allele-specific PCR (KASP) screening of fad2 mutant markers. Twenty-four high-oleic-acid lines of BC4F4 and BC4F5 populations, with morphological features and agronomic traits similar to those of the recurrent parents, were obtained within 5 years. The genetic backgrounds of BC4F5 lines were estimated using the KASP assay, which revealed the genetic background recovery rate was 79.49%—92.31 %. The superior lines raised are undergoing a multi-location test for cultivar registration and release. To our knowledge, this is the first application of single nucleotide polymorphism markers based on the high-throughput and cost-effective KASP assay for detection of fad2 mutations and genetic background evaluation in a peanut breeding program. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13447610
- Volume :
- 69
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Breeding Science
- Publication Type :
- Academic Journal
- Accession number :
- 139462753
- Full Text :
- https://doi.org/10.1270/jsbbs.18107