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Early-life N-arachidonoyl-dopamine exposure increases antioxidant capacity of the brain tissues and reduces functional deficits after neonatal hypoxia in rats.
- Source :
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International Journal of Developmental Neuroscience . Nov2019, Vol. 78, p7-18. 12p. - Publication Year :
- 2019
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Abstract
- • Acute neonatal hypoxia resulted in upregulation of HIF1-α, GPx2 and GPx4 genes expression in rat brains. • Acute neonatal hypoxia led to delay in sensorimotor development and behavioural alterations in adolescent and adult rats. • NADA administration enhanced brain tissue redox capacity both in control rats and in rats exposed to hypoxia. • NADA treatment from P2 to P5 significantly improved sensorimotor development and memory retention in hypoxic rats. • The long-lasting beneficial effects of NADA are mediated, at least in part, by its antioxidant properties. Perinatal hypoxia-ischemia is one of the most common causes of perinatal brain injury and subsequent neurological disorders in children. The aim of this work was to evaluate the potential antioxidant and neuroprotective effects of N-arachidonoyl-dopamine (NADA) in the model of acute neonatal hypoxia (ANH) in rat pups. Male and female Wistar rats were exposed to a hypoxic condition (8% oxygen for 120 min) at postnatal day 2 (P2). Transcription factor HIF1-α and glutathione peroxidases GPx2 and GPx4 gene expression was increased in rat brains in the hypoxic group compared to control 1.5 h but not 4 days after ANH. There were no post-hypoxic changes in reduced (GSH) and oxidised (GSSG) glutathione levels in the brain of rat pups 1.5 h and 4 d after hypoxia. Hypoxic rats displayed retarded performance in the righting reflex and the negative geotaxis tests. ANH resulted in increased ambulation in Open field test and impaired retention in the Barnes maze task under stressful conditions as compared with the control group. Treatment with NADA significantly attenuated the delayed development of sensorimotor reflexes and stress-evoked disruption of memory retention in hypoxic rats but had no effect on the hypoxia-induced hyperactivity. In rats exposed to hypoxia, treatment with NADA decreased GPx2 gene expression and increased GSH/GSSG ratio in whole brains 1.5 h after ANH. These results suggest that the long-lasting beneficial effects of NADA on hypoxia-induced neurobehavioural deficits are mediated, at least in part, by its antioxidant properties. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 07365748
- Volume :
- 78
- Database :
- Academic Search Index
- Journal :
- International Journal of Developmental Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 139347932
- Full Text :
- https://doi.org/10.1016/j.ijdevneu.2019.06.007