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Cloning and identification of the Frigocyclinone biosynthetic gene cluster from Streptomyces griseus strain NTK 97.
- Source :
-
Bioscience, Biotechnology & Biochemistry . Nov2019, Vol. 83 Issue 11, p2082-2089. 8p. - Publication Year :
- 2019
-
Abstract
- Frigocyclinone is a novel antibiotic with antibacterial and anticancer activities. It is produced by both Antarctica-derived Streptomyces griseus NTK 97 and marine sponge-associated Streptomyces sp. M7_15. Here, we first report the biosynthetic gene cluster of frigocyclinone in the S. griseus NTK 97. The frigocyclinone gene cluster spans a DNA region of 33-kb which consists of 30 open reading frames (ORFs), encoding minimal type II polyketide synthase, aromatase and cyclase, redox tailoring enzymes, sugar biosynthesis-related enzymes, C-glycosyltransferase, a resistance protein, and three regulatory proteins. Based on the bioinformatic analysis, a biosynthetic pathway for frigocyclinone was proposed. Second, to verify the cloned gene cluster, CRISPR-Cpf1 mediated gene disruption was conducted. Mutant with the disruption of beta-ketoacyl synthase encoding gene frig20 fully loses the ability of producing frigocyclinone, while inactivating the glycosyltransferase gene frig1 leads to the production of key intermediate of anti-MRSA anthraquinone tetrangomycin. Cloning and identification of the frigocylinone biosynthetic gene cluster provide a deep insight into the biosynthetic pathway and will contribute to development of novel antibiotics via genetic engineering. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09168451
- Volume :
- 83
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Bioscience, Biotechnology & Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 139194694
- Full Text :
- https://doi.org/10.1080/09168451.2019.1638755