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A Human Single-Chain Antibody Specific for Integrin α3β1 Capable of Cell Internalization and Delivery of Antitumor Agents
- Source :
-
Chemistry & Biology . Jul2004, Vol. 11 Issue 7, p897-906. 10p. - Publication Year :
- 2004
-
Abstract
- Selective antitumor chemotherapy can be achieved by using antibody-drug conjugates that recognize surface proteins upregulated in cancer cells. One such receptor is integrin α3β1, which is overexpressed on malignant melanoma, prostate carcinoma, and glioma cells. We previously identified a human single-chain Fv antibody (scFv), denoted Pan10, specific for integrin α3β1 that is internalized by human pancreatic cancer cells. Herein, we describe the chemical introduction of reactive thiol groups onto Pan10, the specific conjugation of the modified scFv to maleimide-derivatized analogs of the potent cytotoxic agent duocarmycin SA, and the properties of the resultant conjugates. Our findings provide evidence that Pan10-drug conjugates maintain the internalizing capacity of the parent scFv and are cytotoxic at nanomolar concentrations. Our Pan10-drug conjugates may be promising candidates for targeted chemotherapy of malignant diseases associated with overexpression of integrin α3β1. [Copyright &y& Elsevier]
- Subjects :
- *DRUG therapy
*CANCER cells
*CANCER
*THERAPEUTICS
Subjects
Details
- Language :
- English
- ISSN :
- 10745521
- Volume :
- 11
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Chemistry & Biology
- Publication Type :
- Academic Journal
- Accession number :
- 13905390
- Full Text :
- https://doi.org/10.1016/j.chembiol.2004.04.018