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Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors.

Authors :
Thomas, Adam M.
Brolih, Sanja
McGouran, Joanna F.
El-Sagheer, Afaf H.
Ptchelkine, Denis
Jones, Morgan
McDonald, Neil Q.
McHugh, Peter J.
Brown, Tom
Source :
Chemical Communications. 10/7/2019, Vol. 55 Issue 78, p11671-11674. 4p.
Publication Year :
2019

Abstract

We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery. [ABSTRACT FROM AUTHOR]

Subjects

Subjects :
*DNA ligases
*OLIGONUCLEOTIDES

Details

Language :
English
ISSN :
13597345
Volume :
55
Issue :
78
Database :
Academic Search Index
Journal :
Chemical Communications
Publication Type :
Academic Journal
Accession number :
138830091
Full Text :
https://doi.org/10.1039/c9cc05476f