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Placental supernatants' enhancement of the metastatic potential of breast cancer cells: is estrogen receptor (ERα) essential for this phenomenon?
- Source :
-
Archives of Gynecology & Obstetrics . Oct2019, Vol. 300 Issue 4, p981-991. 11p. 1 Chart, 4 Graphs. - Publication Year :
- 2019
-
Abstract
- <bold>Purpose: </bold>Pregnancy-associated breast cancer (PABC) is usually diagnosed at an advanced stage in comparison to non-pregnant women. The placenta secretes hormones and cytokines, which affect breast cancer progression. Previously, we demonstrated that human placental secretome facilitates the survival and migration of ERα+ breast cancer cells (BCCL), but pregnant women have a relatively high frequency of ERα-negative tumors. In the current study, we analyzed the effect of placental secretome on ERα-negative BCCL.<bold>Methods: </bold>BCCL [MCF-7(estrogen/progesterone receptor positive (ERα+/PR+), ERα reduced MCF-7 (siRNA, MCF-7 ERα-), HS-578 and BT-549 cells (both ER-/PR-)] were exposed to supernatants (collected from first trimester human placental explants and from control BCCL) or to E2 + P4 (estrogen + progesterone) in placental supernatant concentrations and then tested for cell proliferation (number, cell cycle, PCNA), cell-death, cell migration, STAT3 pathway activation and functionality.<bold>Results: </bold>Silencing ERα in the MCF-7 cells negated the placental supernatant and E2 + P4 enhancement of cell migration (> 130%, p < 0.05), number (> 120%) and survival (~ 130%). However, it had no such effect on MCF-7-ER- migration, which was still elevated in the presence of placental secretome. ER-/PR- BCCL were unaffected by the hormones, but placental secretome significantly elevated their migration (115%), number (140-170%), STAT3 phosphorylation (~ 180%) and BT-549 STAT3 level. These effects were negated by the STAT3 inhibitor.<bold>Conclusions: </bold>Placental supernatant facilitates BCCL malignant characteristics by activating ERα in estrogen responsive cells and STAT3 in ERα- BCCL. This indicates a possible mechanism that may underlie PABC's advanced state and suggests STAT3 pathway as a therapeutic target for PABC. [ABSTRACT FROM AUTHOR]
- Subjects :
- *METASTATIC breast cancer
*ESTROGEN
*ESTROGEN receptors
*CANCER cells
*CELL migration
*CELL cycle
*PROTEIN metabolism
*BREAST tumors
*CARRIER proteins
*CELL lines
*CELL physiology
*CELL motility
*COMPARATIVE studies
*RESEARCH methodology
*MEDICAL cooperation
*PLACENTA
*PREGNANCY complications
*FIRST trimester of pregnancy
*PROGESTERONE
*PROTEINS
*RESEARCH
*EVALUATION research
Subjects
Details
- Language :
- English
- ISSN :
- 09320067
- Volume :
- 300
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Archives of Gynecology & Obstetrics
- Publication Type :
- Academic Journal
- Accession number :
- 138792846
- Full Text :
- https://doi.org/10.1007/s00404-019-05243-4