Crystallization and preliminary crystallographic studies of the mitochondrial F1-ATPase from the yeast Saccharomyces cerevisiae.

A genetically modified (His6-tagged) form of the mitochondrial F1 -ATPase (MW = 370 kDa) has been purified from the yeast Saccharomyces cerevisiae and crystallized in the presence of polythelene glycol (PEG) 6000 as a precipitant, 1 mM NiCl2, 1mM Mg AMP-PNP and 50 μM Mg ADP. X-ray diffraction data were obtained on three separate occasions using synchrotron radiation, with a progression in the quality of the diffraction data, which improved from 3.3 to 3.0 to 2.8 &Aring . On the second occasion, the diffraction was improved by a crystal-annealing procedure. The crystals belong to the monoclinic space group P21, with unit-cell parameters a = 110.6, b = 294.2, c = 190.4 Å, β = 101.6°. The asymmetric unit contains three molecules of yeast F1, with a corresponding volume per protein weight (VM) of 2.8 ų Da-1 and a solvent content of 55%. [ABSTRACT FROM AUTHOR]